BioMarkDiag

CEEX 166/2006

BIOMARKERS USE IN DIAGNOSIS AND CONTROL RESEARCH

UBB

PARTENERS: 

CO      Assoc. Prof. dr. Monica Culea: Project director-UBB
P1       Lecturer Dr. Paraschiva Cherecheş-Panţa: Project responsible-UMF
P2       Assoc. Prof. dr. Adela Pintea: Project responsible-USAMV
P3       Prof. Dr. Eugen Culea: Project responsible-UTCN

PROJECT ABSTRACT:

        The objectives of this project are focused on obtaining rapid and precise, non-invasive or minim invasive new procedures for the diagnosis of the diseases by using of some advanced methodology and technology on biomarkers analysis and metabolomics profiling. The project will correlate the experimental results obtained by different spectroscopic methods (isotopic dilution mass spectrometry, NMR, IR, Raman, UV-VIS, EPR). The interest is to replace the quantitative techniques with rapid, economic and precise profiling techniques, the invasive methods with non-invasive or minim invasive methods The proposed objectives are related to the national and European priority areas. The project will have economical and societal impacts by improving the precocious diagnosis especially in the sensitive group of infants, children, pregnant women and so by the increasing of the population health level. Dissemination will be obtained by national and international publications and clinical monitoring

PROJECT OBJECTIVES:

• Development of minim invasive diagnosis of the inborn metabolic error diseases and the associated biomarkers

• Development and validation of the diagnosis method for cirrhosis and hepatitis by SIM-GC/MS (the caffeine test)

• Identification and selection of biomarkers for diagnosis of some diseases from breath air (extraction methods: SPME, active coal) in comparison with blood and urine samples (extraction method: headspace)

• Selection of the optimal methods for the achievement of non-invasive or minim invasive advanced methods of diagnosis in the monitoring of infants and children

• Communication, dissemination and application of the obtained research results.

  COOPERATIONS WAYS:

  • periodic meetings and discussions

  • e-mail, messenger, telephone communications

  • intern and extern communications and publications

     

   

ACTUAL STAGE OF THE PROJECT:

• Development of a minim invasive method of diagosis for inborn metabolic error diseases(PKU, MSUD); biomarkers (five amino acid); method validation and clinical monitoring of the blood spots (20 µl) for the inborn metabolic error diseases PKU, MSUD;
• Development, validation and clinical application of the diagnosis method for cirrhosis and hepatitis by SIM-GC/MS (the caffeine test)
• Identification and selection of biomarkers for diagnosis of some diseases from breath air (headspace, SPME) in comparison with blood and urine samples (headspace) ; determination of acetone biomarker
• Development of a non invasive method for NO and asthma control determination in breath air  to children
• Development of a non invasive method by SIM-GC-MS for quantitative determination of LKB₄ in exhaled breath
• Communication, dissemination and application of the obtained research results.

• 

                              Blood spots obtained from newborns (20µl)

  

   

PUBLICATIONS:

Non ISI 2007- 2008:
1.       A. Iordache, M. Culea, M. Jimborean, A. Pintea, M. Chiriac, AMINO ACIDS CHARACTERIZATION BY ISOTOPIC DILUTION GC-MS, Journal of Environmental Protection and Ecology- JEPE, in press
2.       M. Culea, M. Popa, P. Cherecheş-Panţa, A. Leucuţa, M. Nanulescu, Clearance-ul cafeinei – metodă pentru evaluarea funcţiei hepatice, Pediatru.ro, 7, 48-50, 2007.
3.       M. Culea, O. Cozar, A. Pintea, ID-GC/MS as a tool for diagnosis of inborn errors of metabolism, Bulletin of University of Agricultural Sciences and Veterinary Medicine - seria Veterinary medicine, 2007, Vol. 64(1-2), pag. 116-120, Print ISSN 1843-5270, Electronic ISSN 1843-5378,

ISI  2008:
1.  M. Culea, R. Fechete, E. Culea, C. Craciun, O. Cozar, COMPARATIVE SPECTROSCOPIC METHODS FOR RAPID DIAGNOSIS, Rev Chimie, in press
2. M. Culea, P. Chereches-Panţa, M. Nanulescu, C. Mesaros, DIAGNOSIS OF HEPATIC DYSFUNCTION DISEASES BY ISOTOPIC DILUTION GC/MS, Rev. Chimie, in press
3. M.Culea, A. Iordache, O. Cozar, GC/MS DETERMINATION OF VOLATILE ORGANIC COMPOUNDS, Rev Chimie, in press
4. M. Culea, A. Iordache, C. Mesaros, AMINOACID PROFILES MONITORING FOR DIAGNOSIS, "Chemické listy" " Journal, 2008,102,s936-938
5. C. Mesaros, M. Culea, E. Culea,  DIAGNOSIS OF CIRRHOSIS BY GC/MS, "Chemické listy" Journal, 2008,102,s961-962
6. M. Culea, E. Culea, P. Chereches-Panta, A. Pintea, LEUKOTRIENE QUANTITATION BY GC-MS, "Chemické listy" Journal, 2008,102,s963-964
7. M. Culea, P. Chereches-Panta, A. Pintea, E. Culea, BIOMARKERS    DETERMINATION BY GC-MS, International Journal of Environmental Analytical Chemistry, in press

Books:
1. M. Culea, Spectrometrie de masa. Pricipii si aplicatii, Ed. Risoprint, Cluj-Napoca, 2008, 302 pag, 978-973-751-655-8